Fig. 2. POLR1D silencing inhibits the cell proliferation of CRC cells and angiogenesis ability of HUVECs.
After SW480 and HT-29 cells were transfected with si-NC or si-POLR1D, qRT-PCR (A) and Western blot (B) were applied to detect the mRNA and protein expressions of POLR1D. The cell proliferation ability was assessed by CCK-8 (C) and clone formation assay (D). Tube formation assay was applied to detect the angiogenesis ability of HUVECs. Scale bar, 20 μm (E). Data were presented as mean ± SD. CRC, colorectal cancer; HUVECs, human umbilical vein endothelial cells. *p < 0.05, **p < 0.01, ***p < 0.001.
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