Primers used for amplification
| Primer | Sequence 5'–3' | Annealing temp (°C) |
Cycles | Ref. | ||
|---|---|---|---|---|---|---|
| MT copy number | ||||||
| MT | Forward | GCCACAACTAGACACATCCACA | 58 | 35 | [23] | |
| Reverse | GGGGGTAATGAAAGAGGCAA | |||||
| Forward | TGCTTCACCACCTTCTTGAT | 60 | 35 | [23] | ||
| Reverse | TGGAAAGCTGTGGCGTGAT | |||||
| Real-time PCR | ||||||
| Forward | TGCTTCACCACCTTCTTGAT | 60 | 35 | [23] | ||
| Reverse | TGGAAAGCTGTGGCGTGAT | |||||
| Forward | CAGCCATGCCACCAAGATC | 61 | 40 | [24] | ||
| Reverse | AAGGGCCGCACAGAATCC | |||||
| Forward | AGAATAGTGGCATGGTGGTAGACG | 61 | 40 | Self design | ||
| Reverse | ACGCAGCGCAGGAGAATCAGA | |||||
| Forward | ATGCCCTCTTTCTAAATCTCGTTCC | 61 | 40 | Self design | ||
| Reverse | TGGTAGGGGCAAATGGTGGTA | |||||
| Forward | GCTTGTTGTAATTGCGGCACGAGGAA | 61 | 40 | Self design | ||
| Reverse | AAGGATCGCGTGAAGGGAAGAAGC | |||||
MT, mitochondrial genome;