Fig. 7. Reversing effect of SO2 in up-regulation of Hippo pathway expression induced by hyperthyroid.
(A–D) Western-Blot results for changes in expressions of MST1, LATS1, and P-YAP in myocardial tissues of rats (with n = 3); *, #, and & meant p < 0.05 vs. the values in the control, L-Thy, and L-Thy + SO2 groups, respectively. (E–H) Western-Blot results for changes in expressions of MST1, LATS1, and P-YAP in H9c2 cardiomyocytes in each group; n = 3. *,#, and & meant p < 0.05 vs. the values in the control, L-Thy, and L-Thy + SO2 groups, respectively. (I, J) Cells were transfected with siRNA negative control (si-NC) or si-YAP1 for 24 h, and the expression of YAP1 was measured by Western blot analysis (I) and quantitatively analyzed (J). n = 3. * meant p < 0.05 vs. the values in the si-NC groups, respectively. (K, L) Western-Blot results for changes in expressions of ERP72 in H9c2 cardiomyocytes in each group; n = 3. * and # meant p < 0.05 vs. the values in the control, and L-Thy groups, respectively. L-Thy, levothyroxine; SO2, sulfur dioxide; HDX, hydrogen deuterium exchange.
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