Fig. 3. The effect of transcription factors on the promoter activity of
SLC40A1. Luciferase activities were measured 48 h after co-transfection of SLC40A1 WT or its variants and various amounts of CREB-1 (A), COUP-TF1 (B), or HLF (C) cDNA into HCT-116 cells. The luciferase activity of each construct was compared with naïve promoter activity. Data shown represent mean ± SD from triplicate wells in a representative experiment. SLC40A1, solute carrier 40A1; WT, wild type; CREB-1, cAMP response element-binding protein-1; COUP-TF1, chicken ovalbumin upstream promoter transcription factor 1; HLF, hepatic leukemia factor. **p < 0.01, ***p < 0.001 vs. naïve promoter activity.
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