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Fig. 2. DNA-protein binding assays to examine the interaction between SLC40A1 promoter variants and transcription factors. Nuclear extracts from HCT-116 cells interacted with biotinylated oligonucleotide of CREB-1 consensus, c.-662C WT, or c.-662T variant (A) and biotinylated oligonucleotide COUP-TF1 consensus, c.-98G WT, or c.-98C variant (B) and biotinylated oligonucleotide of HLF consensus, c.-8C WT, or c.-8G variant (C). DNA-protein binding assay was performed using antibodies against CREB-1, COUP-TF1, or HLF. Data shown represent mean ± SD from three independent experiments. SLC40A1, solute carrier 40A1; WT, wild type; CREB-1, cAMP response element-binding protein-1; COUP-TF1, chicken ovalbumin upstream promoter transcription factor 1; HLF, hepatic leukemia factor; OD, optical density. *p < 0.05, **p < 0.01 vs. WT.
Korean J Physiol Pharmacol 2024;28:113-120
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