Fig. 4. LPS decreased SOCE by downregulating Orai 1 and STIM1 in the sympathetic ganglia.
(A, B) Summary of the relative expression of transcripts encoding STIM1 and Orai1. (C) Immunoblots of Orai1 and STIM1 protein expression in rat SCG 24 h after either vehicle (VEH) or LPS injection in vivo. (D, E) Summary of the relative expression of Orai1 and STIM1 proteins. (F, H) Representative traces of SOCE in SGCs (F) and neurons (H) following treatment with either vehicle or LPS (1 μg/ml, 24 h in vitro). (G, I) Summary of the LPS-induced changes in AUC SOCE in both SGCs and neurons (n = 17–28 cells). The data are presented as means ± SEM. The number of experiments is indicated in parentheses. Unpaired Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001. LPS, lipopolysaccharide; SOCE, store-operated calcium entry; STIM, stromal interaction molecule; SCG, superior cervical ganglia; SGC, satellite glial cell; CPA, cyclopiazonic acid; AUC, area under the curve.
© Korean J Physiol Pharmacol
