Fig. 3. Expression of MLC2, p-MLC2, pp-MLC2 and cGMP-dependent signaling molecules in PAs and MAs.
(A) Western blot analysis of mono- and di-phosphorylated states of MLC2 in PAs and MAs. (B) Summary of total MLC2 expression normalized to β-actin and the levels of p-MLC2 (S19-p) and pp-MLC2 (T18/S19-pp) normalized to the expression of MLC2. (C) Representative immunoblot analysis of sGCα, sGCβ, PKG, ROCK1, ROCK2 and MYPT1 in PAs and Mas. (D) Summary of the protein levels normalized to β-actin. (E) Western blot analysis of phosphorylated states of MLC2 induced by 80K and the effect of pretreatment with Y27632 (10 µM) in PAs and MAs. (F) Summary of normalized levels of p-MLC2 (left panel) and pp-MLC2 (right panel). Data are means ± SD. PA, pulmonary artery; MA, mesenteric artery; PSS, physiological salt solution. *p < 0.05, **p < 0.01, using two-tailed Student’s t-test and one-way ANOVA test.
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