The Korean Journal of Physiology & Pharmacology

Table. 1.

Table 1 List of the antibodies used for immunoblot assay

Antibody	Source	Cat No.	Dilution
Primary antibodies	Source	Cat No.	Dilution
Rabbit anti-Phospho-Myosin Light Chain 2	Cell Signaling Technology, Danvers, MA, USA	3672S	1:1,000
Rabbit anti-Phospho-Myosin Light Chain 2 (Ser19)	Cell Signaling Technology, Danvers, MA, USA	3671S	1:1,000
Rabbit anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19)	Cell Signaling Technology, Danvers, MA, USA	3674S	1:1,000
Rabbit anti-Guanylate Cyclase α subunit (soluble)	Cayman Chemicals, Ann Arbor, MI, USA	160895	1:1,000
Rabbit anti-Guanylate Cyclase β1 subunit (soluble)	Cayman Chemicals, Ann Arbor, MI, USA	160897	1:1,000
Rabbit anti-PKG-1 (C8A4)	Cell Signaling Technology, Danvers, MA, USA	3248	1:1,000
Rabbit anti-ROCK1	Abcam, Cambridge, UK	ab45171	1:1,000
Rabbit anti-ROCK2	Cell Signaling Technology, Danvers, MA, USA	9029S	1:1,000
Rabbit anti-MYPT1	Cell Signaling Technology, Danvers, MA, USA	2634	1:1,000
Mouse anti-β-actin	Sigma-Aldrich, St. Louis, MO, USA	A1978	1:10,000

Secondary antibodies
HRP-linked anti-Mouse IgG	Cell Signaling Technology, Danvers, MA, USA	7076S	1:5,000
HRP-linked anti-Rabbit IgG	Cell Signaling Technology, Danvers, MA, USA	7074S	1:5,000
Rabbit anti-Goat IgG, HRP conjugate	Millipore, Billerica, MA, USA	AP106P	1:5,000