Fig. 5. EX527 reversed the alleviating effects of sweroside on HG-treated HK-2 cells.
(A) Western blot assay for SIRT1 protein expression in HK-2 cells of Ctrl group, HG group, HG + SW (100 µM) group and HG + SW (100 µM) + EX527 group. (B) CCK8 assay for cell viability. (C) ELISA was performed to analyze TNF-α, IL-1β, and VCAM-1 concentration. (D) Detection of ROS in different groups of HK-2 cells by DHE fluorescent staining. Scale bar = 50 µM. (E) Western blot assays for ZO-1, Vimentin, α-SMA, and Snail protein expression. Values are presented as mean ± SD. SW, sweroside; HK-2 cells, human renal tubular epithelial cell; HG, high glucose; Ctrl, control; SIRT1, silent information regulator 2 homolog 1; TNF-α, tumor necrosis factor-α; IL, interleukin; ROS, reactive oxygen species. ***p < 0.001, compared with the control group; ##p < 0.01, ###p < 0.001, compared with the HG group; ^^p < 0.01, ^^^p < 0.001, compared with the HG + SW (100 µM) group.
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