Fig. 3AThe inhibitory effects of mecheliolide, costunolide and dehydrocostus lactone on endogenous TMEM16A-mediated CaCC currents in LA795 lung adenocarcinoma cells.(A) The representative traces of endogenous TMEM16A-mediated CaCCs in LA795 cells recorded using voltage-command shown in
. Dotted lines indicate zero current amplitude. (B) Representative endogenous TMEM16A-mediated CaCC current traces in LA795 cells (recorded using the voltage protocol shown in
Fig. 2E before and after the effects of 100 μM mecheliolide, costunolide and dehydrocostus lactone were stabilized. The effects of MONNA (10 μM) are also shown. (C) Bar graph showing the inhibitory effect of 100 μM mecheliolide, costunolide and dehydrocostus lactone and 10 μM MONNA on endogenous TMEM16A-mediated CaCCs. Values are presented as mean ± SEM. CaCC, Ca
2+-activated Cl
– channel. *p < 0.05,
vs. the current amplitudes in the absence of drugs; n = 5 for each experimental group.
