Fig. 3. The effects of mecheliolide, costunolide and dehydrocostus lactone on the I-V relationship of TMEM16A-mediated CaCC currents in CHO cells.
(A) Voltage step protocol. 1.5-s step pulses between –80 and +80 mV with increments of 20 mV from a holding potential of 0 mV followed by a –100 mV step. (B) Representative patch-clamp recordings of whole-cell TMEM16A-mediated CaCC currents evoked by 300 nM Ca2+ in the absence (control) or presence of 100 μM mecheliolide, costunolide and dehydrocostus lactone in the bath medium. The effects of 10 μM MONNA on the currents are shown in the right panels. (C–E) The mean normalized current-voltage relationships of whole-cell TMEM16A-mediated CaCC currents. n = 5 cells, per group. CaCC, Ca2+-activated Cl- channel; CHO, Chinese hamster ovary; I–V, current-voltage.
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