Fig. 2. The inhibitory effects of mecheliolide, costunolide and dehydrocostus lactone on recombinant TMEM16A-medicated CaCC currents in CHO cells.
Representative patch-clamp recordings of current traces at 300 nM Ca2+ in the presence of 100 μM mecheliolide (A), costunolide (B), dehydrocostus lactone (C), and 10 μM MONNA (D) in the bath medium. (E) Whole-cell TMEM16A-medicated CaCC currents were stimulated with a 4-s ramp voltage protocol from −80 mV to +80 mV. The holding potential was set to 0 mV. (F) Bar graph showing the inhibitory effects of mecheliolide (100 μM), costunolide (100 μM), dehydrocostus lactone (100 μM), and MONNA (10 μM) on TMEM16A-medicated CaCCs at +80 mV. Values are presented as mean ± SEM. Current traces recorded at the time points indicated by the letters (a, b). CaCC, Ca2+-activated Cl- channel; CHO, Chinese hamster ovary. *p < 0.05, vs. the current amplitudes in the absence of drugs; n = 5 cells, per group.
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