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Fig. 2. DSF has little effect on apoptosis and cell viability.
After 24 h of treatment, the DCs were stained with Annexin V-FITC and PI. (A) The quadrants of dot plot indicate live cells (annexin V/PI), the cells in early apoptosis (annexin V+/PI), late apoptosis (annexin V+/PI+), and necrosis (annexin V/PI+). (B) Statistical analysis was performed based on the results of the four experiments conducted under the same conditions. A trypan blue exclusion assay was performed after 3 days of treatment. (C, D) As a result of four experiments under the same conditions, the net number of live and dead cells was counted. The viability was calculated by the ratio of live cells to total cells. Results are presented as mean ± SD, and statistical significance was performed by two-way ANOVA followed by Tukey’s multiple comparisons test (B, C) or Dunnett’s multiple comparisons test (D). * indicates p < 0.05 compared to control DCs (DSF 0 µM). DSF, Disulfiram; DCs, dendritic cells; LPS, lipopolysaccharide; FITC, fluorescein isothiocyanate; PI, propidium iodide.
Korean J Physiol Pharmacol 2023;27:471-479
© Korean J Physiol Pharmacol