Fig. 1. The effect of DSF on the cellular metabolic activity.
(A) DCs. (B) BMs. (C) Spleen cells. Cells were cultured in a 96-well plate with 0 to 1 µM of DSF with or without LPS. Concentrations of LPS were 0.1 µg/ml on DCs and BMs, and 1 µg/ml on spleen cells. 1 µg/ml of DOX was used as a positive control on DCs. The MTT assay was performed at 3 days after the treatment. The optical density (O.D.) was measured at 570 nm by using a microplate reader. Results are presented as mean ± SD, and statistical significance was performed by two-way ANOVA followed by Dunnett’s multiple comparisons test. *, **, **** indicate p < 0.05, 0.01, 0.0001 respectively compared to control cells (DSF 0 µM). DSF, Disulfiram; DCs, dendritic cells; BMs, bone marrow cells; LPS, lipopolysaccharide; DOX, doxorubicin; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylltetrazolium bromide.
© Korean J Physiol Pharmacol
