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Fig. 6. KIF15 knockdown induces apoptosis in NPC cells through activation of p53.
CNE-2 (A) or 5-8F (B) cells were transfected with sip53 or negative control (NC) for 48 h, the mRNA expression of p53 was detected. KIF15-shRNA/CNE-2 (C, E) or KIF15-shRNA/5-8F (D, F) cells were transfected with sip53 or NC. The flow cytometric analysis was detected. The mRNA expression of bax (G, H), bcl-2 (I, J) and bax/bcl-2 (K, L) were detected. The protein expression of cleaved-caspase 3 was detected in CNE-2 (M) and 5-8F (N) cells. Values are presented as mean ± SD. KIF15, kinesin superfamily member 15; NPC, nasopharyngeal carcinoma. *p < 0.05 and **p < 0.01 vs. the KIF15-shRNA-NC group, n = 5.
Korean J Physiol Pharmacol 2023;27:457-470
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