Fig. 3. KIF15 knockdown induces apoptosis in NPC cells.
(A, B) The flow cytometric analysis was detected in KIF15-shRNA/CNE-2 and ScrshRNA/CNE-2 cell lines. (C, D) The flow cytometric analysis was detected in KIF15-shRNA/5-8F and ScrshRNA/5-8F cell lines. The mRNA expression of Bax (E), Bcl-2 (G), and Bax/Bcl-2 (I) were detected in KIF15-shRNA/CNE-2 and ScrshRNA/CNE-2 cell lines. The mRNA expression of Bax (F), Bcl-2 (H), and Bax/Bcl-2 (J) were detected in KIF15-shRNA/5-8F and ScrshRNA/5-8F cell lines. The protein expression of cleaved-caspase 3 was detected in CNE-2 (K) and 5-8F (L) cells. Values are presented as mean ± SD. KIF15, kinesin superfamily member 15; NPC, nasopharyngeal carcinoma; ns, no significance. *p < 0.05 and **p < 0.01 vs. the shcontrol group, n = 5.
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