Fig. 1. Identification of memantine and ifenprodil as N-retinylidene-N-retinylethanolamine (A2E) degraders.
(A, B) ARPE-19 cells were incubated with 10 μM A2E-BDP for 24 h and then treated with memantine or ifenprodil (10, 25, and 50 μM) for 48 h. A2E-BDP fluorescence intensity was measured using a microplate reader. (C, D) Cell viability of memantine and ifenprodil in ARPE-19 cells. ARPE-19 cells were treated with various concentrations of the compounds. After incubation, cell viability was calculated using an EZ-Cytox assay kit. Results are presented as the mean ± SD. (n = 3). *p < 0.05, **p < 0.01 vs. A2E-BDP.
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