Fig. 6. MiR-103-3p enhances myoblasts proliferation.
C2C12 cells were transfected with scRNA, siTWF1, miR-103-3p mimic (miR-103), or antimiR-103 and cultured for 24 h. (A) The cells undergoing DNA replication were labeled with EdU (green), and Hoechst 33342 (blue) was used for nuclei staining. Scale bar: 50 μm. (B) The proportion of EdU-positive cells was measured using ImageJ software. (C, D) Flow cytometry with a scatter plot of cell cycle analysis after transfection with scRNA or miR-103-3p mimic (miR-103). (E) Relative expression levels of PCNA, CCNB1, and CCND1 were analyzed by qRT-PCR and normalized to U6 levels. The values are shown as relative ratios, with the intensity of the scRNA control set to one. All data are shown as the mean ± SEM (n > 3), where the level of significance is represented as *p < 0.05; **p < 0.01; ***p < 0.001 vs. scRNA. TWF1, Twinfilin-1; EdU, ethynyl deoxyuridine; qRT-PCR, real-time qualitative polymerase chain reaction; ns, no significance.
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