Fig. 5. MiR-103-3p facilitates F-actin accumulation and nuclear YAP1 localization.
C2C12 cells were transfected with scRNA, siTWF1, or miR-103-3p mimic (miR-103) and cultured for 24 h. (A) The transfected cells were labeled with FITC-phalloidin (green) for F-actin staining and Hoechst 33342 (blue) for nucleus staining. Representative images from three independent experiments are shown. Scale bar: 25 μm. (B) Phalloidin intensities were analyzed by the ImageJ program and presented as relative ratios, with the intensity of the scRNA control set to one. (C) Representative immunoblots of YAP1 and phosphorylated YAP1 (pYAP1) protein in the cytoplasmic and nuclear fractions are shown. α-Tubulin and Lamin B were used as the cytoplasm and nucleus markers, respectively. β-Actin was used for loading control. (D) The immunoblot intensities were normalized to the amount of β-Actin or α-Tubulin, and the values are shown as relative ratios, with the intensity of the normalized scRNA control set to one. All data are shown as the mean ± SEM (n > 3), where the level of significance is represented as **p < 0.01; ***p < 0.001 vs. scRNA. F-actin, filamentous actin; YAP1, Yes-associated protein 1; TWF1, Twinfilin-1; ns, no significance.
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