Fig. 4. MiR-103-3p impairs myogenic differentiation and myotube formation.
C2C12 cells were transfected with scRNA, siTWF1, miR-103-3p mimic (miR-103), or antimiR-103 and differentiated for five days. (A) The cells were stained with an anti-MyHC antibody (green) and Hoechst 33342 nuclear dye (blue). Scale bar: 50 μm. (B) MyHC-positive areas, differentiation indices, fusion indices, and myotube widths were analyzed as described in the Materials and Methods. All data are shown as the mean ± SEM (n > 3), where the level of significance is represented as ***p < 0.001 vs. scRNA. MyHC, myosin heavy chain; TWF1, Twinfilin-1; ns, no significance.
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