Fig. 3. MiR-103-3p inhibits the expressions of myogenic factors.
C2C12 cells were transfected with scRNA, siTWF1, miR-103-3p mimic (miR-103), or antimiR-103 and differentiated for three days. (A) Representative immunoblots from three independent experiments on day three of differentiation. (B) The immunoblot intensities were normalized to the amount of β-Actin. The values are shown as relative ratios, with the intensity of the normalized scRNA control set to one. All data are shown as the mean ± SEM (n > 3), where the level of significance is represented as **p < 0.01; ***p < 0.001 vs. scRNA. TWF1, Twinfilin-1; ns, no significance.
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