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Fig. 7. MiR-4636 depletion inhibited the anti-cancer effects of AP1S3 knockdown in GC. HGC-27 and AGS cells were transfected with si-AP1S3, si-NC, inhibitor, inhibitor-NC, or si-AP1S3+inhibitor. (A) Western blotting revealed the expression of AP1S3 protein. (B) CCK-8 assay was used to analyze the cell proliferative capacity. (C) Western blotting confirmed the expression of Bax and Bcl-2. (D) Wound-healing assay showed cell migration ability. (E) Colony-formation assay was used for proliferation analysis. Values are presented as mean ± SD. ×200. AP1S3, adaptor protein complex 1 sigma 3; GC, gastric cancer. *p < 0.05, **p < 0.01 in contrast to si-NC; #p < 0.05, ##p < 0.01 in contrast to inhibitor-NC; &p < 0.05, &&p < 0.01 in contrast to si-AP1S3+inhibitor.
Korean J Physiol Pharmacol 2023;27:197-208
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