Fig. 5. MiR-4636 inhibition reversed the inhibitory effects of LINC00562 silencing on GC cell survival.
HGC-27 and AGS cells were transfected with si-lnc, si-NC, inhibitor, inhibitor-NC, or si-lnc+inhibitor. (A) RT-qPCR was used to assess the expression level of miR-4636. (B) CCK-8 assay tested the proliferative capacity of cells. (C) Western blotting confirmed the expression of Bax and Bcl-2. (D) Wound-healing assay showed cell-migration ability. (E) Colony-formation assay was used for proliferation analysis. Values are presented as mean ± SD. ×200. GC, gastric cancer. *p < 0.05, **p < 0.01 in contrast to si-NC; #p < 0.05, ##p < 0.01 in contrast to inhibitor-NC; &p < 0.05, &&p < 0.01 in contrast to si-lnc+inhibitor.
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