Fig. 5. Nonfunctional PLCδ1 mutants on PIP2 level have no effect on TRPC4 currents.
(A) Representative whole cell current recordings of HEK293 cells co-expressed with TRPC4β and PLCδ1 (K30A/K32A) (A) or PLCδ1 (H311A) (B). Left panel: Time course of currents at ±100 mV every 10 sec; Right panel: I-V relationship for selected time points. Stippled lines indicate zero currents. Application of 100 nM (-)-Englerin A (EA) are indicated. The pipette solution contained 100 nM free Ca2+. (C) Schematization of PLCδ1. The mutation sites are indicated with arrow. (D) Summaries of peak current densities at –60 mV induced by EA. PLCδ1 mutants had no effect on TRPC4β currents as the PLCδ1 non-expressing cells. Data are expressed as mean ± SEM. TRPC, transient receptor potential canonical; PLC, phospholipase C. **p < 0.01, ***p < 0.001 by t-test. The numbers in parentheses refer to cell numbers.
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