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Fig. 4. Channel calcium-activated PLCδ1 accelerates PIP2 depletion. (A, B) PIP2 depletion from plasma membrane to cytosol after (-)-Englerin A stimulation. Imaging was performed in the cells expressing TRPC4β and CFP-PH domain in the absence (A) or presence of PLCδ1 (B). PIP2 changes are monitored by CFP-PH domain. Left panel: Images of CFP-PH domain for selected time points; Right panel: Line scan graph of each image. The line scanned regions are indicated by dashed line. Scale bars, 10 μm. TRPC, transient receptor potential canonical; PLC, phospholipase C; PIP2, phosphatidylinositol 4,5-bisphosphate.
Korean J Physiol Pharmacol 2023;27:187-196
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