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Fig. 3. Ca2+-dependent activation of PLCδ1 occurs in physiological intracellular calcium range. (A, C, E) Representative whole-cell current recordings of HEK293 cells co-expressed with TRPC4β in the absence or presence of PLCδ1 using 50 nM (A), 100 nM (C), and 500 nM (E) free Ca2+ recording pipette solutions. Left panel: Time course of currents at ±100 mV every 10 sec; Left panel: I-V relationship for selected time points. Stippled lines indicate zero currents. Applications of 100 nM (-)-Englerin A (EA) are indicated. (B, D, F) Summaries of peak current densities at –60 mV induced by EA. The PLCδ1 inhibited TRPC4 currents when using 100 nM and 500 nM [Ca2+]i recording solutions. Data are expressed as mean ± SEM. TRPC, transient receptor potential canonical; PLC, phospholipase C. **p < 0.01, ***p < 0.001 by t-test. The numbers in parentheses refer to cell numbers.
Korean J Physiol Pharmacol 2023;27:187-196
© Korean J Physiol Pharmacol