Fig. 2. PLCδ1 inhibits TRPC4β currents.
(A) Rapamycin-induced translocation of CFP-FKBP-PLCδ1 to the plasma membrane. CFP-FKBP-PLCδ1 and Lyn-FRB were co-expressed in HEK293 cells, and 50 nM rapamycin was used. The line scanned region is indicated by dashed line. Scale bars, 10 μm. (B, C) Representative whole-cell current recordings of HEK293 cells co-expressed with TRPC4β, FKBP-PLCδ1 in the absence (B) or presence of Lyn-FRB (C). Left panel: Time course of currents at ±100 mV every 10 sec; Right panel: I-V relationship for selected time points. Stippled lines indicate zero currents. Applications of 50 nM rapamycin and 100 nM (-)-Englerin A (EA) are indicated. The pipette solution contained 100 nM free Ca2+. (D) Summaries of peak current densities at –60 mV induced by rapamycin and EA. Rapamycin-induced PLCδ1 translocation to plasma membrane significantly reduced TRPC4β currents. Data are expressed as mean ± SEM. TRPC, transient receptor potential canonical; PLC, phospholipase C. *p < 0.05 by t-test. The numbers in parentheses refer to cell numbers.
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