Fig. 1. TRPC4β and PLCδ1 colocalize together.
(A, C) Localizations of TRPC channel and PLCδ enzyme. Upper panel: Channel with PLCδ1; Lower panel: Channel with PLCδ3. ECFP-tagged channel and EYFP-tagged PLCδ were co-expressed in HEK293 cells. The line scanned position is indicated by arrow in overlay images. Line scan graph shows TRPC4β colocalized with PLCδ1, but not with PLCδ3. Original magnification, ×63. (B, D) Summaries of FRET efficiency in the same expression conditions. The numbers in parentheses refer to cell numbers. (E) Representative blots of Co-IP experiments. HEK293 cells were co-expressed with Flag-tagged channel and EYFP-tagged PLCδ. Proteins from each condition were subjected to immunoprecipitation using anti-GFP antibody and probed with anti-Flag antibody. TRPC4β interacts with PLCδ1 directly but not with another subtype. TRPC5 interacts with neither. Data are expressed as mean ± SEM. TRPC, transient receptor potential canonical; PLC, phospholipase C; Co-IP, Co-immunoprecipitation.
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