Fig. 5. KLF9 promotes the expression of TLR2 in macrophages.
(A) Q-PCR analysis of Tlr2 mRNA expression in myocardial tissues from WT mice after MI or sham operation. (B) Immunoblot analysis of TLR2 protein level in myocardial tissues from WT mice after MI or sham operation. (C) Q-PCR analysis of Tlr2 mRNA expression in myocardial tissues from Klf9 KO or WT mice at day 7 after MI. (D) Immunoblot analysis of TLR2 protein level in myocardial tissues from Klf9 KO or WT mice at day 7 after MI. (E) ChIP analysis of KLF9 enrichment level at the Tlr2 promoter in WT macrophages treated with HMGB1 (100 ng/ml) or control PBS (Ctrl) for 12 h. Data presented as mean ± SD. MI, myocardial infarction; KO, knockout; WT, wild-type; HMGB1, high-mobility group box 1. *p < 0.05; **p < 0.01; ***p < 0.001. Unpaired Student’s t-test or ANOVA was performed.
© Korean J Physiol Pharmacol
