Fig. 4. KLF9 promotes inflammatory responses triggered by high-mobility group box 1 (HMGB1) in macrophages.
(A) ELISA of IL-1β, IL-6 and TNF-α production in BMDMs from Klf9 KO or WT mice after HMGB1 (100 ng/ml) treatment for 12 h. (B) ELISA of IL-1β, IL-6 and TNF-α production in supernatants of BMDMs transfection with Klf9 siRNA or control siRNA followed by treatment with HMGB1 (100 ng/ml) for 12 h. (C) Immunoblot analysis of phosphorylation levels of proteins in lysates of BMDMs from Klf9 KO or WT mice after HMGB1 (100 ng/ml) treatment. (D) Immunoblot analysis of phosphorylation levels of proteins in lysates of BMDMs transfected with the control siRNA or Klf9 siRNA after stimulated with HMGB1 (100 ng/ml). Data presented as mean ± SD. KO, knockout; WT, wild-type; IL, interleukin; TNF-α, tumor necrosis factor-α; BMDM, bone marrow derived macrophage. *p < 0.05; **p < 0.01; ***p < 0.001. Unpaired Student's t-test was performed.
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