Fig. 4. RD restored ovarian functions in DOR rats through the FOXO1/KLOTHO axis.
DOR rats were injected with sh-FOXO1, LV-KLOTHO, and their negative controls, in addition to intervention with a high dose RD for 2 weeks. (A, B) The expression of KLOTHO in ovarian tissues was determined by qRT-PCR (A) and Western blotting (B). (C) Vaginal smear test was used to measure the estrous cycles of the rats. (D) Ovarian index. (E) Ovarian H&E staining, and quantitative histograms of primordial, mature, and atretic follicles. (F–H) The levels of FSH (F), LH (G), and E2 (H) were tested by ELISA assay. (I) Ovarian TUNEL staining was used to detect cell apoptosis. (J) The expression of Bcl-2 and Bax was investigated using Western blotting. Values are presented as mean ± SD. DOR, diminished ovarian reserve; RD, Rehmannioside D; FSH, Follicle-stimulating hormone; LH, luteinizing hormone; E2, estradiol; FOXO1, Forkhead Box O1. n = 10. *p < 0.05.
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