Fig. 3. FOXO1 enhanced KLOTHO transcription by binding to the KLOTHO promoter.
(A, B) The expression of KLOTHO in ovarian tissues was measured by qRT-PCR (A) and Western blotting (B). (C) The binding site of FOXO1 and the KLOTHO promoter was predicted by Jaspar database. (D) ChIP assay was used to detect the binding of FOXO1 to the KLOTHO promoter. (E, F) qRT-PCR (E) and Western blotting (F) were used to investigate the expression of KLOTHO in rat ovarian tissues. Values are presented as mean ± SD. DOR, diminished ovarian reserve; RD, Rehmannioside D; FOXO1, Forkhead Box O1; ChIP, Chromatin immunoprecipitation. n = 10. *p < 0.05.
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