Fig. 2. RD treatment relieved DOR progression in rats by upregulating FOXO1.
(A, B) qRT-PCR (A) and Western blotting (B) were implemented to investigate the expression of FOXO1 in ovarian tissues. DOR rats were injected with sh-FOXO1 or its negative control and administrated with a high dose of RD for 2 weeks. (C, D) qRT-PCR (C) and Western blotting (D) were used to determine FOXO1 in ovarian tissues. (E) The estrous cycles of rats were measured using vaginal smears. (F) Ovarian index. (G) Ovarian H&E staining, and the number of primordial, mature, and atretic follicles. (H–J) The levels of FSH (H), LH (I), and E2 (J) were examined by ELISA assay. (K) Cell apoptosis in rat ovaries was investigated with TUNEL staining. (L) Western blotting was used to test the expression of Bcl-2 and Bax. Values are presented as mean ± SD. DOR, diminished ovarian reserve; RD, Rehmannioside D; FSH, Follicle-stimulating hormone; LH, luteinizing hormone; E2, estradiol; FOXO1, Forkhead Box O1. n = 10. *p < 0.05.
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