Fig. 2. Knockdown of Rab5 in MDA-MB-231 cells by small interfering RNA.
(A) Effect of siRNA concentration and incubation time on transfected with siRNAs (n = 5), cells were stained with Block-iT Alexa Fluor Red, magnification 100×. (B) mRNA level of Rab5 in MDA-MB-231 cells transfected with siRNAs (n = 5). (C) Protein level of Rab5 in MDA-MB-231 cells transfected with siRNAs (n = 3). (D) mRNA and (E) protein levels of Rab5 in MDA-MB-231 cells transfected with empty or Rab5-containing vectors. Data was represented as mean ± SD. *p < 0.05 vs. control.
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