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Fig. 6. The tumorigenic role of miR-665 may be achieved by targeting PTPRB 3ʹUTR. LUAD cells were transfected with PTPRB-overexpressing vectors (OE-PTPRB), OE-NC, miR-665 mimic (mimic), mimic-NC, and OE-PTPRB+mimic. (A) Cell proliferation was analyzed using the CCK-8 assay. (B) Western blot analysis of anti-Bax and anti-Bcl-2 expression in the transfected cells. (C) Colony formation was determined using colony formation assay (×200). *p < 0.05, **p < 0.01 vs. OE-NC; #p < 0.05, ##p < 0.01 vs. mimic-NC; &p < 0.05, &&p < 0.01 vs. OE-PTPRB+mimic. Values are presented as mean ± SD. LUAD, lung adenocarcinoma; OD, optical density; PTPRB, protein tyrosine phosphatase receptor type B.
Korean J Physiol Pharmacol 2023;27:131-141
© Korean J Physiol Pharmacol