Fig. 2. Augmentation of circ_0129047 expression suppresses LUAD tumors in vitro and in vivo.
circ 0129047-overexpressing vectors (OE-circ) and empty vectors were transfected into PC9 and Calu-3 cells (OE-NC). (A) RT-qPCR analysis of circ_0129047 expression 48 h later. (B) Cell proliferation assay using the CCK-8 assay. (C) Expression of apoptosis-related proteins (Bcl-2 and Bax) determined using Western blotting. (D) Colony formation was assessed using colony formation assay (×200). (E) Xenograft tumor growth assay. Values are presented as mean ± SD. LUAD, lung adenocarcinoma; OD, optical density. **p < 0.01 vs. OE-NC.
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