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Fig. 2. Knockdown of lnc-ABCA12-3 inhibited the proliferation and glycolysis of esophageal squamous cell carcinoma (ESCC) cells. (A) After transfection with sh-ABCA12-3, lnc-ABCA12-3 expression was measured by RT-PCR. (B) The viability of ESCC cells were assessed by CCK-8 assay. (C, D) The proliferation was detected by EdU assay. Scale bar, 20 μm. (E–G) The contents of ATP, glucose uptake, and lactic acid production were tested by the corresponding commercial kits. Data were presented as mean ± SD. **p < 0.01 vs. sh-NC group.
Korean J Physiol Pharmacol 2023;27:61-73
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