Fig. 5. SO2 reduces cardiomyocyte apoptosis in diabetic rats.
(A) TUNEL staining of myocardial tissue of the rats in the Control, DC, DC + SO2 and SO2 groups (apoptosis-positive cells in TUNEL staining are in brown, and they are indicated by black arrows), n = 3. Images were acquired at 10 × 40 magnification; (B) Quantitative results of TUNEL-stained positive cells in the myocardial tissue of the rats in the respective group, **p < 0.01 vs. Control. ##p < 0.01 vs. DC; (C–G) Western blot detection of the expressions of Bax, Bcl2, Cleaved Caspase3, Caspase3, Cleaved Caspase9, Caspase9 in the myocardial tissue of the rats in the respective group. n = 3, *p < 0.05 vs. Control; #p < 0.05 vs. DC; (H, I) Detection of mRNA expression levels of Bax and Caspase3 in the myocardial tissue by RT-qPCR, **p < 0.01 vs. Control. ##p < 0.01 vs. DC, GAPDH is taken as the internal parameter. Values are presented as mean ± SD. SO2, sulfur dioxide; DC, diabetic cardiomyopathy.
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