Fig. 3. Sulfur dioxide (SO₂) can reduce the formation of myocardial collagen fibers in type II diabetic rats. (A) Masson staining results of myocardial tissue of the rats in the Control, DC, DC + SO₂, and SO₂ groups. 10 × 40 magnification field of view (blue-stained part is collagen fibers, and black arrows are collagen fibers), n = 3; (B) Fraction of Masson-stained collagen volume, **p < 0.01 vs. Control; ^##p < 0.01 vs. DC. (C, D) Western blot analysis of the protein expression changes of MMP3, MMP8, MMP13, and CollagenIII in the myocardial tissue of the rats in the respective group. n = 3, *p < 0.05 vs. Control; ^#p < 0.05 vs. DC. (E) RT-qPCR detection of the expression of Col3a1 in the myocardial tissue of the rats. n = 3, **p < 0.01 vs. Control; ^##p < 0.01 vs. DC, Take GAPDH as reference. Values are presented as mean ± SD deviation. DC, diabetic cardiomyopathy.

Korean J Physiol Pharmacol 2022;26:541-556 https://doi.org/10.4196/kjpp.2022.26.6.541

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