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Fig. 5. Effects of the mGluR5 antagonist MPEP and the mGluR1 antagonist LY367385 on 0.1 mM [Mg2+]o-induced neurotoxicity in cultured pure rat hippocampal neurons. Cells were exposed to HEPES-buffered HBSS containing 0.1 mM Mg2+ and 10 µM glycine for 24 h in the presence or absence of MPEP (25 µM) or LY367385 (100 µM). Neuronal cell survival was measured by MTT reduction assay at 12 days after culture. Cell survival was shown as a percentage of control value. The absorbance of formazan that had formed in non-treated cells grown in culture medium (control) represented 100% viability. (A) Bar graph showing MTT reduction in HEPES-buffered HBSS (control) (vehicle, n = 6; 0.1 mM Mg2+, n = 6), MPEP (vehicle, n = 6; 0.1 mM Mg2+, n = 6), LY367385 (vehicle, n = 6; 0.1 mM Mg2+, n = 6)-treated cells. (B) Representative phasecontrast photomicrographs showing cultured pure rat hippocampal neurons at 24 h following co-treatment of mGluR antagonists with 0.1 mM [Mg2+]o solution for 11 days in culture. Data are expressed as means ± SEM. mGluR, metabotropic glutamate receptor; MPEP, 6-Methyl-2-(phenylethynyl) pyridine; HBSS, Hank’s balanced salt solution; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. ++p < 0.01 relative to respective vehicle (non-paired Student’s t-test), *p < 0.05 relative to respective control (ANOVA with Bonferroni test).
Korean J Physiol Pharmacol 2022;26:531-540
© Korean J Physiol Pharmacol