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Fig. 4. Effects of TRPC channel inhibitors such as SKF96365 and flufenamic acid on 0.1 mM [Mg2+]o-induced [Ca2+]i spikes. (A) 0.1 mM [Mg2+]o induced synchronized and repetitive [Ca2+]i spikes within 30 sec at day 11.5. (B) SKF96365 (10 µM) almost completely inhibited 0.1 mM [Mg2+]o-induced [Ca2+]i spikes. (C) flufenamic acid (100 µM) inhibited 0.1 mM [Mg2+]o-induced [Ca2+]i spikes. (D, E) Graph summarizing the frequency (D) and the area under the curve (E) of 0.1 mM [Mg2+]o-induced [Ca2+]i spikes in non-treated (control, n = 19), SKF96365-treated (n = 22), and flufenamic acid-treated (n = 20) cells. Relative spike frequencies or area under curves (2nd /1st: drug or non-treatment; 3rd / 1st: 0.1 mM [Mg2+]o wash) were shown as a ratio of an initial [Ca2+]i spike frequency and area under curves for 1st 0.1 mM [Mg2+]o treatment. Data are expressed as means ± SEM. **p < 0.01 relative to respective control (ANOVA with Bonferroni test), ##p < 0.01 relative to respective control and flufenamic acid (ANOVA with Bonferroni test).
Korean J Physiol Pharmacol 2022;26:531-540
© Korean J Physiol Pharmacol