Fig. 4. miR-23b targeting of SMAD3.
(A) Target genes of miR-23b have been predicted using bioinformatics. (B) The mRNA expression of WEE1, CNOT2, HMGA2, and SMAD3 in vascular smooth muscle cells (VSMCs) after miR-23b mimic transfection. (C, D) The protein expression of WEE1, CNOT2, HMGA2, and SMAD3 in VSMCs after miR-23b mimic transfection. The representative image was displayed (C), followed by statistical analysis in triplicate (D). (E) Complementary sequences of Wt/Mut-SMAD3 3UTR and miR-23b. (F) Dual-luciferase reporter assay demonstrating that SMAD3 is an miR-23b target. (G) In VSMCs, the level of SMAD3 protein was decreased by the miR-23b mimic and increased by lactate. Values in this figure are presented with mean ± SD. n = 3. NC, negative control. *p < 0.05, **p < 0.01, and ***p < 0.001.
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