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Fig. 3. miR-23b mimic attenuated the effects of lactate on vascular smooth muscle cell (VSMC) phenotype switch. (A) qRT-PCR analysis of the expression of miR-23b in VSMCs after transfection with a miR-23b mimic. (B–F) VSMCs cells were treated with 4 mM lactate with or without overexpression of a miR-23b mimic. (B) VSMC cell viability was determined using CCK-8. (C) The Tunel assay was used to determine the apoptosis of VSMSCs (magnification 200×). (D) Using JC-1 staining, the mitochondrial membrane potential of VSMCs was determined. (E) The migration of VSMCs was determined using a transwell assay, and the quantitative results were presented (magnification 400×). (F) The effects of lactate on the protein levels of α-SMA, SM22, SM-MHC, vimentin, and collagen 1 in VSMCs were attenuated using a Western blot assay. Values in this figure are presented with mean ± SD. n = 3. NC, negative control. *p < 0.05, **p < 0.01, and ***p < 0.001.
Korean J Physiol Pharmacol 2022;26:519-530
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