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Fig. 9. Effects of PP1β knockdown on phenotypic changes. Cells were transfected with control (lane 1) or siRNA of PP1β plasmids as described in the legend for . The expression of PP1β (A, upper panel) and cytosolic pMLC20 level (A, lower panel) were determined using supernatant protein (60 μg). p-values were obtained by unpaired Student’s t-tests (n = 4). Active renin secretion was determined before and after stimulation by ML-7 (6 × 10−5 M) for 1 h each, and secreted renin was measured by ELISA (B). PP1β, protein phosphatase 1β; pMLC20, phosphorylated 20 kDa myosin light chain. p-values were obtained either by paired Student’s t-tests (within groups) or by ANOVA (between groups) (n = 6).
Korean J Physiol Pharmacol 2022;26:479-499
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