Fig. 8. Effects of MYLK knockdown on phenotypic changes.
Cells were transfected with control or siRNA of MYLK plasmids as described in the legend of . Expression of MYLK and the cytosolic pMLC20 level (A) were determined using supernatant protein (60 μg). p-values were obtained by unpaired Student’s t-tests (n = 3). Active renin secretion from control and knockdown cells was determined by incubating cells in DMEM + 10% FBS for 1 h (B, n =6). pMLC20, phosphorylated 20 kDa myosin light chain; DMEM, Dulbecco’s modified eagle medium; FBS, fetal bovine serum. p-values by unpaired Student’s t-tests.
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