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Fig. 7. Effects of N-cad expression on pMLC20 and PP1β. Cells were cultured to 100% confluence and maintained for two more days in the presence of 10% FBS (lane 1) or in the presence of both FBS and IGF-1 antibody (10 μg/ml) (lane 2). Proteins in the supernatant (40 μg) were resolved by Western blotting as described above (n = 4). pMLC20, phosphorylated 20 kDa myosin light chain; PP1β, protein phosphatase 1β; FBS, fetal bovine serum; IGF-I, insulin-like growth factor-I. p-values were obtained by unpaired Student’s t-tests.
Korean J Physiol Pharmacol 2022;26:479-499
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