Table. 6.

Effects of various agents on active renin secretion

Agent Action n Effect on active renin secretion (E/C)
Ionomycin (10−5 M) Ca2+ ionophore 6 0.57 ± 0.12*
ANG II (10−8 M) Phospholipase activator 7 0.54 ± 0.16*
U73122 (3 × 10−5 M) PLC inhibitor 5 4.23 ± 1.63*
U73343 (3 × 10−5 M) Inactive analogue of U 73122 5 1.19 ± 0.38
Ophiobolin A (3 × 10−5 M) CaM inhibitor 6 3.76 ± 1.22*
K252a (10−5 M) Broad spectrum protein kinase inhibitor 6 2.33 ± 0.93*
Butanedione monoxime (25 mM) MLCK inhibitor 5 4.35 ± 0.85*
GF-109203X (3 × 10−6 M) PKC inhibitor 6 0.97 ± 0.15
Y-27632 (3 × 10−5 M) Rho kinase inhibitor 8 1.34 ± 0.11*
Calyculin A (2 × 10−7 M) Protein phosphatase 1 & 2A 6 0.77 ± 0.12*
LY294002 (5 × 10−5 M) Phosphatidylinositol-3 kinase inhibitor 5 0.61 ± 0.13*
Wortmanin (10−6 M) Phosphatidylinositol-3 kinase inhibitor 5 0.54 ± 0.09*
SB216763 (2 × 10−5 M) GSK-3β inhibitor 6 1.89 ± 0.17*
Blebbistatin (10−6 M) Myosin ATPase inhibitor 8 1.21 ± 0.37

Values represent means ± SD. Cells were grown in DMEM + 10% FBS to 100% confluence. On day 2 postconfluence, cells were incubated in 1 ml fresh DMEM + 10% FBS for 1 h (C) followed by incubation in medium containing one of the above agents (E). Concentrations in parentheses are the concentrations tested. (E/C) values significantly greater than 1.0 or less than 1.0 indicate stimulation or inhibition of renin secretion, respectively. DMEM, Dulbecco's modified eagle medium; FBS, fetal bovine serum. *p < 0.05.

Korean J Physiol Pharmacol 2022;26:479-499 https://doi.org/10.4196/kjpp.2022.26.6.479
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