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Fig. 6. Effects of SRF knockout on phenotypic changes. Cells were transfected with control plasmids (A, lane 1) or SRF HDR CRISPR/Cas9 plasmids (A, lane 2) as described in the legend of . Then, the expression of each protein was determined in the supernatant fraction (80 μg). Both control and knockout cells were incubated with ML-7 (6 × 10−5 M) before and after stimulation for 1 h each, and secreted active renin was measured by ELISA (B, n = 5). SRF, serum response factor; HDR, homology directed repair; CRISPR, clustered regularly interspaced short palindromic repeats. p-values were obtained either by paired Student’s t-tests (within groups) or by ANOVA (between groups).
Korean J Physiol Pharmacol 2022;26:479-499
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