Table. 5.

Effects of ML-7, forskolin + IBMX, and calyculin A on inactive renin secretion from postconfluent cells

Control ML-7 Forskolin Calyculin A
Prorenin (ng/well/h) 81.5 ± 7.6 21.4 ± 1.1*,** 36.3 ± 9.7*,** 155.2 ± 20.7*

Values represent means ± SD from 6 wells. On day 2 postconfluence, cells were incubated in DMEM + 10% FBS alone (Control) or containing ML-7 (6 × 10−5 M), forskolin (3 × 10−5 M) + IBMX (10−4 M), or calyculin A (2 × 10−7 M) for 1 h. Levels of secreted prorenin were determined with the use of mouse prorenin/renin total antigen ELISA kit from Molecular Innovations. DMEM, Dulbecco's modified eagle medium; FBS, fetal bovine serum. p-values were calculated by ANOVA. *p < 0.001 vs. Control; **p < 0.001 vs. Calyculin A. No significant difference between ML-7 and Forskolin.

Korean J Physiol Pharmacol 2022;26:479-499 https://doi.org/10.4196/kjpp.2022.26.6.479
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