Table. 3.

Effects of confluence, FBS, and insulin-like growth factor-I (IGF-I) on active renin secretion

Pre-confluence Postconfluence
DMEM + 10% FBS DMEM + 10% FBS IGF-I IGF-I + Antibody
Renin secretion (ng/well/h) 10.5 ± 3.47 31.6 ± 1.25* 73.9 ± 5.72** 20.1 ± 1.70***

Values represent means ± SD from five wells. As4.1 cells were cultured in DMEM + 10% FBS to pre-confluence (70%–80% confluence) or day 2 postconfluence (postconfluence). Alternatively, cells were cultured in DMEM + IGF-I (2.6 × 10−10 M) or DMEM + IGF-I (2.6 × 10−10 M) + antibodies against IGF-I (10 μg/ml) to day 2 postconfluence (postconfluence). Culture media were replaced with fresh media and incubated for 1 h to measure the rate of renin secretion by ELISA. DMEM, Dulbecco's modified eagle medium; FBS, fetal bovine serum. p-values were calculated using the unpaired Student’s t-test. *p < 0.001 DMEM + 10% FBS at Pre-confluence vs. DMEM + 10% FBS at Postconfluence; **p < 0.001 DMEM + 10% FBS at Postconfluence vs. DMEM + IGF-I at Postconfluence; ***p = 0.001 DMEM + IGF-I at Postconfluence vs. DMEM + IGF-I + Antibody at Postconfluence.

Korean J Physiol Pharmacol 2022;26:479-499 https://doi.org/10.4196/kjpp.2022.26.6.479
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